The story is told first from the outside, through Mr. Utterson, a reserved London lawyer. His kinsman Enfield describes a strange scene: a small, repellent man who calmly trampled a child in the street and then, to settle the matter, produced a cheque signed by a respected name. The name is that of Utterson's friend Dr. Henry Jekyll, and the man is one Edward Hyde, to whom Jekyll's will leaves everything. Disturbed, Utterson sets out to learn who Hyde is and what hold he has over his friend.
Utterson's unease deepens into alarm when Hyde is seen battering an elderly gentleman, Sir Danvers Carew, to death in the street with ape-like fury. Hyde vanishes, Jekyll produces a letter claiming the danger is past, and for a time the doctor seems restored, sociable, and well. Then he abruptly shuts himself away. The cooperative investigation, the murder, and Jekyll's swings between calm and dread are all watched from a puzzled distance, the reader knowing only as much as the lawyer does.
The crisis comes in Jekyll's locked laboratory. His servant Poole fetches Utterson in terror, convinced his master has been murdered and that the cringing figure now hiding in the cabinet is someone else. They break down the door and find Hyde dead by his own hand in Jekyll's clothes, with no Jekyll anywhere to be found. Two documents are left behind, a narrative from the late Dr. Lanyon and a full statement by Jekyll, and only these can explain what the watchers have seen.
Jekyll's statement reframes the whole story from within. From youth he had concealed his pleasures and felt himself divided, and his studies led him to the conviction that a human being is not truly one but truly two. He compounds a drug that lets the lower nature take separate shape as Edward Hyde, smaller, younger, and purely evil. At first the transformation thrills him: he can sin as Hyde and wake innocent as Jekyll, his reputation untouched and his conscience asleep.
The arrangement turns against him. Hyde's cruelties grow, he murders Carew, and the change begins to come unbidden, so that Jekyll wakes as Hyde without taking the drug and must dose himself merely to stay himself. As his supply of the salt fails and cannot be replaced, the balance tips for good toward the worse self. Writing under the last of the powder, knowing Hyde will soon return for the final time, Jekyll lays down his pen and brings his own unhappy life to an end.